The precise haplotype structure of the recombinant population was defined with unprecedented accuracy and resolution. Many genomic regions show a clustering of recombination events including significant hot spots. Recombination rate, SFP distribution, and segregation in this population are not uniform. One hundred fifteen single gene intervals were identified. The genetic linkage map had a total length of 422.5 cM, with 676 informative SFP markers representing intervals of ~0.6 cM. Allelic variation at these loci was measured in a recombinant inbred line population, which defined the location of 815 recombination breakpoints. Around 16,000 single feature polymorphisms (SFPs) were detected in ~8,000 of the ~26,000 genes represented on the array. The genotypes of two accessions of Arabidopsis were compared by using a 400,000 feature exon-specific oligonucleotide array. Genotyping several hundred thousand loci in a single assay by hybridizing genomic DNA to oligonucleotide arrays provides a powerful technique to improve precision linkage mapping. Recombinant populations were the basis for Mendel's first genetic experiments and continue to be key to the study of genes, heredity, and genetic variation today.
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